GgaEX0011328 @ galGal4

Exon Skipping

Gene

ENSGALG00000007943 | lasp-2

Description

nebulette non-muscle isoform [Source:RefSeq peptide;Acc:NP_989819]

Coordinates

chr2:18216122-18225701:+

Coord C1 exon

chr2:18216122-18216214

Coord A exon

chr2:18220307-18220399

Coord C2 exon

chr2:18225530-18225701

Length

93 bp

Sequences

Splice sites

3' ss Seq

TCGTAATATTTATTGTGCAGATT

3' ss Score

6.33

5' ss Seq

AATGTGTGC

5' ss Score

0.36

Exon sequences

Seq C1 exon

GTGTTTTATAAGAAGGTGGTAGGAGCTGGCACAGCTGTTAAAGAGACTCCAGAGATTGAAAGAGTAAAGAAAAATCAGCAGAATATTAGTTCG

Seq A exon

ATTAAATACAAAGAAGAAACTCAGCATGCAACTCCTATTTCTGATCCACCCGAACTAAGGAGAATCAAGGAAAACCAGAAGAATATAAGCAAT

Seq C2 exon

GTTAAATATCATGAAGATTTTGAGAAGACAAAGGGCAGGGGTTTCACGCCAGTGGTGGATGATCCTATAACAGAGCGGGTGCGGAAAAACACCCAAGTTGTAAGTGATGCAGCATATAAAGGGGTGCACCCACATATAGTTGAAATGGATAGAAGACCTGGAATAATTGTTG

VastDB Features

Vast-tools module Information

Secondary ID

ENSGALG00000007943_MULTIEX1-23/26=22-C2

Average complexity

C3

Mappability confidence:

77%=100=80%

Protein Impact

Alternative protein isoforms (No Ref)

No structure available

Features

Disorder rate (Iupred):

C1=0.581 A=0.935 C2=0.428

Domain overlap (PFAM):

C1:

PF0088013=Nebulin=PD(21.7=16.1),PF0088013=Nebulin=PU(59.3=51.6)

A:

PF0088013=Nebulin=PD(33.3=29.0),PF0088013=Nebulin=PU(66.7=51.6)

C2:

PF0088013=Nebulin=PD(37.9=19.0),PF0088013=Nebulin=WD(100=41.4)

Main Inclusion Isoform:

ENSGALP00000012888

Main Skipping Isoform:

ENSGALT00000012901fB1254

Other Inclusion Isoforms:

NA

Other Skipping Isoforms:

NA

Other assemblies

galGal3

Conservation

Human

(hg38)

chr10:20823208-20823300

HIGH PSI

HsaEX6087795

(NEBL)

Human

(hg19)

chr10:21112137-21112229

HIGH PSI

HsaEX6087795

(NEBL)

Mouse

(mm10)

chr2:17380223-17380309

Mouse

(mm9)

chr2:17304487-17304579

LOW PSI

MmuEX6099111

(Nebl)

Rat

(rn6)

chr17:84174942-84175035

Cow

(bosTau6)

chr13:22422100-22422192

HIGH PSI

BtaEX6064029

(NEBL)

Zebrafish

(danRer10)

chr9:23004469-23004561

ALTERNATIVE

DreEX0049986

(neb)

Fruitfly

(dm6)

No conservation detected

Primers PCR

Suggestions for RT-PCR validation

F:

AAGAAGGTGGTAGGAGCTGGC

R:

TTCCGCACCCGCTCTGTTATA

Band lengths:

170-263

Functional annotations

(Submit an annotation)

There are 0 annotated functions for this event

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]

Special

GgaEX0011328 @ galGal4

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]