Special

DmeEX6000003 @ dm6

Exon Skipping

Gene
FBgn0025836 | RhoGAP1A
Description
The gene Rho GTPase activating protein at 1A is referred to in FlyBase by the symbol DmelRhoGAP1A (CG40494, FBgn0025836). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (4 unique). Gene sequence location is X:127449..140340. Its molecular function is described by: Rho guanyl-nucleotide exchange factor activity; GTPase activator activity. It is involved in the biological process described with: positive regulation of GTPase activity; positive regulation of Rho protein signal transduction; Golgi organization; negative regulation of cell size. 13 alleles are reported. No phenotypic data is available. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages.
Coordinates
chrX:138770-139524:+
Coord C1 exon
chrX:138770-138930
Coord A exon
chrX:139067-139206
Coord C2 exon
chrX:139275-139524
Length
140 bp
Sequences
Splice sites
3' ss Seq
ATATATTTCTGTTCATTCAGACG
3' ss Score
5.84
5' ss Seq
CTGGTGAAT
5' ss Score
3.53
Exon sequences
Seq C1 exon
TTGAGTTTAAGTTGGCTGACAGAAACAACGCAGCCGAAGTCAATTAAACTTACTGAGACCTTAGAGCTCGGCTGTAGCTTCCGTTTTATACCAGGCGAGCTCTTCCGCGGCAGCACAAAGCCAGGAGCCCTCTTCGGTGCCAAAATGAGTCAAGTATTAAA
Seq A exon
ACGTGAAAAACGCGACATTCCGTTCATCATAGGCGCCTGCATTCGGGAGGTAGAGCGGCGTGGGATGCTGGAGGTTGGCTGCTACCGCGTCAGCGGTTCCGCTTCTGATTTGGCGAAGCTGAAGAAGGCCTTTGAGTCTG
Seq C2 exon
ATGCCTATGAGGCGGAGCAGTTGCTACGCGAGGTGGACATCCATTCGGTCACTGGCATTCTGAAAACTTTTTTAAGGGAGCTCCCCGAGGCCCTTTTCACGGATCAACTTTATCCACGATTTTTCGACACATTCAGCGCCTTTAGCAACAACAACGAATCCACACGAATCAACGAGCTTCTAAAAGTTTTTGAGGAGTTGCCACAGGCCAACAAAGCCTCCATTACTTCGATATTAGATCATTTAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0025836-'27-20,'27-18,30-20=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):

C1:
NO
A:
PF0062022=RhoGAP=PU(25.5=83.3)
C2:
PF0062022=RhoGAP=FE(52.9=100),PF069266=Rep_Org_C=PU(58.3=58.3)


Main Inclusion Isoform:
FBpp0309061


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0111832, FBpp0111833, FBpp0309060


Other Skipping Isoforms:
NA
Associated events
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAGACCTTAGAGCTCGGCT
R:
TGCTAAAGGCGCTGAATGTGT
Band lengths:
256-396
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)