DmeEX6008512 @ dm6
Exon Skipping
Gene
FBgn0085421 | Epac
Description
The gene Exchange protein directly activated by cAMP is referred to in FlyBase by the symbol DmelEpac (CG34392, FBgn0085421). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 2R:6759715..6798941. Its molecular function is described by: guanyl-nucleotide exchange factor activity. It is involved in the biological process described with: regulation of Rap protein signal transduction. 20 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: fertile; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, at stages throughout the pupal period.
Coordinates
chr2R:6768579-6769359:-
Coord C1 exon
chr2R:6769308-6769359
Coord A exon
chr2R:6768926-6769023
Coord C2 exon
chr2R:6768579-6768665
Length
98 bp
Sequences
Splice sites
3' ss Seq
AACTATGGCTGCCTCTCCAGGTT
3' ss Score
6.83
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
Exon sequences
Seq C1 exon
AAAAACAAGGAGTTAATGAATGACATATTCACCAACTGCAAGTTGAAGAACG
Seq A exon
GTTTTGGACCGAGCGTACAAGCGACAGCCGCAGCCACATCGCCCACGAAACGACCCCTCAGCCCCGACCACCCAAACCCAGCGCTGCCCATCACCGAG
Seq C2 exon
ACACCGAGTCCTGCTATGAACCGCATGGGCTGGGCCCTACGCACACTACTCGTCGCCGACAACTCGAGCTGTCTGAAGGATCGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0085421-'15-12,'15-11,16-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.818 C2=0.069
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=PD(3.2=16.7)
A:
NO
C2:
PF0061016=DEP=PU(7.2=17.2)
Main Inclusion Isoform:
FBpp0302889
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0111548, FBpp0302890, FBpp0302891
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACATATTCACCAACTGCAAGT
R:
CTTGCGATCCTTCAGACAGCT
Band lengths:
119-217
Functional annotations
There are 1 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)