Special

DmeEX6014695 @ dm6

Exon Skipping

Gene
FBgn0035533 | Cip4
Description
The gene Cdc42-interacting protein 4 is referred to in FlyBase by the symbol DmelCip4 (CG15015, FBgn0035533). It is a protein_coding_gene from Dmel. It has 12 annotated transcripts and 12 polypeptides (10 unique). Gene sequence location is 3L:4322491..4364102. Its molecular function is described by: GTPase activating protein binding; lipid binding; phospholipid binding; protein binding. It is involved in the biological process described with 13 unique terms, many of which group under: macromolecule localization; plasma membrane organization; membrane organization; mesoderm development; germ cell development. 36 alleles are reported. The phenotypes of these alleles manifest in: multicellular structure; organism; cell junction; cellular anatomical entity; integumentary system. The phenotypic classes of alleles include: increased mortality during development; phenotype; increased mortality; neuroanatomy defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages.
Coordinates
chr3L:4327233-4336319:-
Coord C1 exon
chr3L:4336269-4336319
Coord A exon
chr3L:4327773-4327917
Coord C2 exon
chr3L:4327233-4327702
Length
145 bp
Sequences
Splice sites
3' ss Seq
ATGCTACCATTCCATTACAGATT
3' ss Score
7.84
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
GCGCCTAGTGAAAAACTACCAGCCCAAAAAGAAGGAGGAGGAAGACAATGA
Seq A exon
ATTCACATCGGTGCAAGCGTTCCGCAATCTGCTCAAGGAGGTGGGCGATCTGGCGGGACAGCGCGAGGTGGTGTCCGAGTCCCTGCAGCTGCAGATCATTGCGGGAGTGACGCTTCTGTCCAAGACATTGCGCGAGGAACGCAAG
Seq C2 exon
AAATGCCTTAGCGATGGTGCCACCCTGCAGCAGAACCTCACCACACAGCTCTCCTCGCTGGACCGGGCCAAGCGGAACTACGAGAAGGCCTACCGTGACTCGGAGAAGGCGGTGGACAGCTATAAGCGGGCAGACATGGACCTCAATCTCAGCCGGGCCGAGGTGGAGCGCTACAAGAACGTGATGACGTCCAAGATCCAGCAGTCGGACGATGCGAAGAACGAGTACGCTAACCAGCTACAGAAGACGAACAATCTGCAGCAGCAACACTACAGCATGCTGCTGCCCTCGGTCCTCAATCGGCTGCAGGAGCTGGACGAGAAACGCACCCGTGGCTTCAGGGAGTTCATTGTGGGAGCGGCGGATGTGGAGTCATCGGTGGCGCCAATCATAGCCCGCTGCATGGAGGGTATCGTGAAGGCCGGCGAGTCCATCAACGAAAAGGAGGATACCTTCAAAGTCATAGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0035533-'13-10,'13-9,17-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.631 A=0.020 C2=0.185
Domain overlap (PFAM):

C1:
PF0061118=FCH=FE(18.5=100)
A:
PF0061118=FCH=PD(30.4=57.1),PF0157614=Myosin_tail_1=PU(5.8=16.3)
C2:
PF0157614=Myosin_tail_1=PD(92.7=80.9)


Main Inclusion Isoform:
FBpp0112051


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0073195, FBpp0297231, FBpp0297232, FBpp0297233, FBpp0297491, FBpp0297492, FBpp0300521, FBpp0300522, FBpp0305747, FBpp0305748


Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
HIGH PSI
Zebrafish
(danRer10)
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAGCCCAAAAAGAAGGAGG
R:
ATCGTCCGACTGCTGGATCTT
Band lengths:
247-392
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)