DmeEX6024766 @ dm6
Exon Skipping
Gene
FBgn0011747 | Ank
Description
The gene Ankyrin is referred to in FlyBase by the symbol DmelAnk (CG1651, FBgn0011747). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (1 unique). Gene sequence location is 4:115074..129849. Its molecular function is described by: cytoskeletal anchor activity; spectrin binding; ion channel binding. It is involved in the biological process described with: signal transduction; protein localization to plasma membrane. 10 alleles are reported. The phenotype of these alleles manifest in: ovariole. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period.
Coordinates
chr4:116686-118931:-
Coord C1 exon
chr4:118838-118931
Coord A exon
chr4:118555-118779
Coord C2 exon
chr4:116686-118501
Length
225 bp
Sequences
Splice sites
3' ss Seq
TAATCTATACCCTTTATTAGGTT
3' ss Score
5.44
5' ss Seq
AAGGTAAAC
5' ss Score
8.14
Exon sequences
Seq C1 exon
AAATACTACCCAATGAAATGAGCTGTATTGAATTGACTGAAATCGGGCACAAACCCGATAACGTTGTTATTGCCCGATCCCAAGTTCATTTAGG
Seq A exon
GTTTCTGGTGTCGTTCTTGGTAGATGCACGTGGCGGATCCATGCGCGGATATCGGCACAATGGTGTGCGCATTATCGTACCTCCAAAGGCCTGCGCTGAACCAACACGAATAACATGTCGATATGTTAAGCCGCAAAGGGTAGTCAACCCTCCGCCTTTAATGGAGGGTGAAGCACTTGTGAGTCGCATTTTGGAAATGTCACCTGTTGACGGAATGTTTTTAAG
Seq C2 exon
TCCAATAACTTTAGAAGTTCCACACTATGGCACCCTTAGAAAAAACGAGCGCGAAATTATTATACTCCGGTCCGACAATGGAGAAAGTTGGCGTGAGCATAACTTATATAAAGACATAATAGGTGAAGATATAAACCAAACGGAGGAATTTCATTCGGATCGCATCGTTCGGATCGTTACTCAAAATGTTCCGCACTTTTTTGCTGTTGTTTCGCGAGTTCGTCAGGAAGTGCACGTTATTGGACCTGATGGTGGAACCGTTTTCTCTACAGCAGTGCCCCAGGTCAAAGCTATATTTCCGCCTCATGCTTTAACTAAAAAAATTCGAGTCGGTCTTCAGGCTCAGTCAGTAGACCTGGTTGAATGTTCTAAACTGCTCGGTCAAGGGGTCGCTGTCTCACCCGTAGTAACTGTGGAGCCACGCCGTCGAAAATTTCACAAAGCTATTACATTAAGCATTCCAGCTCCAAAAGCATGTACAAATAGTATGGTTAATGCAT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0011747-'19-18,'19-16,20-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.013 C2=0.015
Domain overlap (PFAM):
C1:
PF0079115=ZU5=PU(1.0=3.1)
A:
PF0079115=ZU5=FE(71.4=100)
C2:
PF0079115=ZU5=PD(26.7=5.1),PF0053117=Death=WD(100=15.6)
Main Inclusion Isoform:
FBpp0088238
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0088239, FBpp0088240, FBpp0289724
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCCGATCCCAAGTTCATTT
R:
TGGGGCACTGCTGTAGAGAAA
Band lengths:
306-531
Functional annotations
There are 1 annotated functions for this event
PMID: 26024478
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926), exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)