Special

DmeEX6024050 @ dm6

Exon Skipping

Gene
FBgn0028671 | Vha100-1
Description
The gene Vacuolar H[+] ATPase 100kD subunit 1 is referred to in FlyBase by the symbol DmelVha100-1 (CG1709, FBgn0028671). It is a protein_coding_gene from Dmel. It has 11 annotated transcripts and 11 polypeptides (5 unique). Gene sequence location is 3R:29131193..29137980. Its molecular function is described by: proton-transporting ATPase activity, rotational mechanism; ATPase binding; photoreceptor activity; calmodulin binding. It is involved in the biological process described with 7 unique terms, many of which group under: anatomical structure development; system development; response to stimulus; cellular metabolic process; ion transmembrane transport. 21 alleles are reported. The phenotypes of these alleles manifest in: cellular anatomical entity; vacuole; membrane-bounded organelle; organelle; cytoplasm. The phenotypic classes of alleles include: stress response defective; increased mortality during development; phenotype; sensory perception defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3R:29132126-29133961:+
Coord C1 exon
chr3R:29132126-29132302
Coord A exon
chr3R:29132376-29132504
Coord C2 exon
chr3R:29133861-29133961
Length
129 bp
Sequences
Splice sites
3' ss Seq
CTTTCTTGTCTGCTGCTCAGGCC
3' ss Score
6.75
5' ss Seq
GAGGTAAGC
5' ss Score
9.85
Exon sequences
Seq C1 exon
CTCAATCCCGATGTCAACGCTTTCCAGAGGAAGTTCGTCAACGAGGTGCGCCGCTGCGATGAGATGGAGCGCAAGCTGCGTTACCTGGAGAAGGAGATCAAGAAGGACGGCATCCCCATGTTGGACACCGGGGAGAGTCCCGAGGCCCCGCAGCCCCGCGAGATGATCGACCTGGAG
Seq A exon
GCCACCTTTGAGAAGCTGGAGAACGAGCTGAGGGAGGTGAATCAGAACGCCGAGGCCCTGAAGCGCAACTTTCTGGAGCTGACCGAGCTGAAGCATATTTTGCGCAAAACCCAAGTGTTCTTCGATGAG
Seq C2 exon
CAAGAAGGCGGCGTAAACCAAACGACCGAGTCGATGACCCGCGCCTTGATCACGGACGAGGCGCGCACCGCTGGTGCCTCCATGGGTCCCGTACAGCTCGG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0028671-'5-6,'5-2,6-6=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.353 A=0.000 C2=0.618
Domain overlap (PFAM):

C1:
PF0149614=V_ATPase_I=FE(7.1=100)
A:
PF0149614=V_ATPase_I=FE(5.1=100)
C2:
PF0149614=V_ATPase_I=FE(4.1=100)


Main Inclusion Isoform:
FBpp0290864


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0084743, FBpp0084744, FBpp0084745, FBpp0084746, FBpp0084748, FBpp0084749, FBpp0084750, FBpp0290865, FBpp0307388


Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Primers PCR
Suggestions for RT-PCR validation
F:
CGCTTTCCAGAGGAAGTTCGT
R:
CTGTACGGGACCCATGGAGG
Band lengths:
256-385
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)