Special

DmeEX6010970 @ dm6

Exon Skipping

Gene
FBgn0034013 | unc-5
Description
The gene unc-5 is referred to in FlyBase by the symbol Dmelunc-5 (CG8166, FBgn0034013). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (1 unique). Gene sequence location is 2R:15340472..15368300. Its molecular function is described by: netrin receptor activity involved in chemorepulsion; netrin receptor activity. It is involved in the biological process described with 6 unique terms, many of which group under: multicellular organism development; anatomical structure morphogenesis; developmental process; regulation of locomotion; plasma membrane bounded cell projection morphogenesis. 29 alleles are reported. The phenotypes of these alleles manifest in: plasma membrane bounded cell projection; cell projection; dorsal vessel primordium; visual system neuron; filopodium. The phenotypic classes of alleles include: increased mortality; cell migration defective; phenotype; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages.
Coordinates
chr2R:15358009-15360468:+
Coord C1 exon
chr2R:15358009-15358290
Coord A exon
chr2R:15358579-15358749
Coord C2 exon
chr2R:15360415-15360468
Length
171 bp
Sequences
Splice sites
3' ss Seq
TTTCCGTTTTCATCCCACAGTCA
3' ss Score
10.4
5' ss Seq
CAGGTACGT
5' ss Score
10.65
Exon sequences
Seq C1 exon
ATATTCGCAAGTCCTTCAACCAGTCGCCCACCTCGCTGCGCCTGGAGCTGGGCAGTCGGGCGGAACTGCGCTGCGAACCACCCGGCGGTTTCCCCGAACCGAAGCTCACCTGGCACAAAAACAACGCGGTCATCACGGCGGACAGCGAGCCGGGGATCACCGTTTCGGCCGGCACACTCATCTTCCGCCAGGTGGCCCTGCAGCATATGGCCAACTATAGCTGCAGTGCGGAGAATATCGCTGGCAGACGCGTCTCCGATTCCGCCGTGCTCATCGTTTATG
Seq A exon
TCAACGGTGGCTGGAGCACCTGGAGTCCATGGCGCGAATGCAAGTGTGCGGGCAAGCCCAGCCAGGGAAGGAAGCGTTCGCGCACCTGCAACAATCCGATGCCATTGAATGGGGGCGCCCAGTGTCCGGGCCCCCAGATCCAGAAGTCCGCCGACTGCGCCGCATGTCCAG
Seq C2 exon
AGGACACTCAAATCGTGAGCCCTGATGGATTTGACATTTCGTCGAGTAAGCGCA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0034013-'10-8,'10-7,11-8=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.053 A=0.172 C2=0.105
Domain overlap (PFAM):

C1:
PF0767911=I-set=WD(100=94.7)
A:
NO
C2:
NO


Main Inclusion Isoform:
FBpp0086514


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0309017


Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
HIGH PSI
Chicken
(galGal4)
HIGH PSI
Chicken
(galGal3)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
Zebrafish
(danRer10)
HIGH PSI
Primers PCR
Suggestions for RT-PCR validation
F:
GTTTCCCCGAACCGAAGCTC
R:
TGCGCTTACTCGACGAAATGT
Band lengths:
249-420
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)