DmeEX0006982 @ dm6
Exon Skipping
Gene
FBgn0028671 | Vha100-1
Description
The gene Vacuolar H[+] ATPase 100kD subunit 1 is referred to in FlyBase by the symbol DmelVha100-1 (CG1709, FBgn0028671). It is a protein_coding_gene from Dmel. It has 11 annotated transcripts and 11 polypeptides (5 unique). Gene sequence location is 3R:29131193..29137980. Its molecular function is described by: proton-transporting ATPase activity, rotational mechanism; ATPase binding; photoreceptor activity; calmodulin binding. It is involved in the biological process described with 7 unique terms, many of which group under: anatomical structure development; system development; response to stimulus; cellular metabolic process; ion transmembrane transport. 21 alleles are reported. The phenotypes of these alleles manifest in: cellular anatomical entity; vacuole; membrane-bounded organelle; organelle; cytoplasm. The phenotypic classes of alleles include: stress response defective; increased mortality during development; phenotype; sensory perception defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3R:29132376-29135175:+
Coord C1 exon
chr3R:29132376-29132504
Coord A exon
chr3R:29133861-29133961
Coord C2 exon
chr3R:29135049-29135175
Length
101 bp
Sequences
Splice sites
3' ss Seq
CCACCATTCACAATTTCTAGCAA
3' ss Score
5.38
5' ss Seq
CGGGTACGT
5' ss Score
9.5
Exon sequences
Seq C1 exon
GCCACCTTTGAGAAGCTGGAGAACGAGCTGAGGGAGGTGAATCAGAACGCCGAGGCCCTGAAGCGCAACTTTCTGGAGCTGACCGAGCTGAAGCATATTTTGCGCAAAACCCAAGTGTTCTTCGATGAG
Seq A exon
CAAGAAGGCGGCGTAAACCAAACGACCGAGTCGATGACCCGCGCCTTGATCACGGACGAGGCGCGCACCGCTGGTGCCTCCATGGGTCCCGTACAGCTCGG
Seq C2 exon
CTTCGTGGCTGGCGTCATCCTGAGGGAGCGACTGCCGGCCTTCGAGCGGATGTTGTGGCGCGCCTGCAGGGGTAACGTCTTCCTGCGACAGGCGATGATTGAGACGCCGCTGGAGGATCCCACCAAT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0028671_MULTIEX1-2/3=C1-C2
Average complexity
ME(1-2[99=100])
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.618 C2=0.070
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(5.1=100)
A:
PF0149614=V_ATPase_I=FE(4.1=100)
C2:
PF0149614=V_ATPase_I=FE(5.1=100)
Main Inclusion Isoform:
FBpp0084744
Main Skipping Isoform:
FBpp0084743
Other Inclusion Isoforms:
FBpp0084746, FBpp0084749, FBpp0290864, FBpp0290865, FBpp0307388
Other Skipping Isoforms:
FBpp0084745, FBpp0084748, FBpp0084750
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GCCACCTTTGAGAAGCTGGAG
R:
CCAGCGGCGTCTCAATCATC
Band lengths:
242-343
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)