Special

DmeEX6023674 @ dm6

Exon Skipping

Gene
Description
The gene without children is referred to in FlyBase by the symbol Dmelwoc (CG5965, FBgn0010328). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (5 unique). Gene sequence location is 3R:27256595..27264055. Its molecular function is described by: zinc ion binding; chromatin binding; protein binding; DNA-binding transcription factor activity, RNA polymerase II-specific. It is involved in the biological process described with: ecdysone biosynthetic process; regulation of transcription, DNA-templated; regulation of transcription by RNA polymerase II. 30 alleles are reported. The phenotypes of these alleles manifest in: circulatory system; cellular anatomical entity; organism subdivision; intracellular non-membrane-bounded organelle; intracellular organelle. The phenotypic classes of alleles include: lethal; lethal - all die before end of pupal stage; phenotype; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3R:27256595-27257838:-
Coord C1 exon
chr3R:27257709-27257838
Coord A exon
chr3R:27257544-27257643
Coord C2 exon
chr3R:27256595-27257480
Length
100 bp
Sequences
Splice sites
3' ss Seq
CTTAACTTTTTTTCAAACAGATG
3' ss Score
9.45
5' ss Seq
ATGGTTAGT
5' ss Score
7.07
Exon sequences
Seq C1 exon
ACCGTGGAGGAGCACATGCAGTTATCCTTCTCGCACATAATGAAGCACTGGAAGCGCTCATCACAGAATTCCAAAGTTCCTGGCTCACGAAACGTGCTCTTACGATTCTATCCACCGCAGGCGGGTCTGG
Seq A exon
ATGCCAATCCGCGGAAGAAGAAAGTCTACGAGCAGCAGGAGAATGAAGAGAATCCGCTGCGCTGTCCCGTCCGCCTTTACGAATTTTATCTCTCCAAATG
Seq C2 exon
CCCGGAGAGCGTGAAGACCCGCAATGATGTCTTTTATCTGCAGCCGGAGCGCTCGTGTGTGCCCGACTCGCCGGTTTGGTACTCGACGCAGGCTCTGGGGCAGGACGCACTGCAGCGGATGCTGCACCGCGTCAAAATGGTCAAGGAAATCAACATAGCGCTACTAACGACTTAATGTTTAGTTAGGCAGTTATATCATTGTGTACGTGCACGGCTTGTCCTGCAATGACACCCGGAGGACGATTCTTCGGCTTGGTGCTTAGCACATGTTTGTTTGCGGGCAAAACCAAATTGGACTAACCAAGGAGCGAGAGTTGTGCTATATTATAAATTGTTTTAAACTTATTTGTACGCATATTTCGATTGTCTCTAGTTGCGATAACGCTGATGGAGGTGACCTTGTATAAAATGTGAACGCCAACCAAGCACATCTCACTTTCGATTGACTAAACTTAATCTAGGAATCAAAAAAATCCAAGCAGCAATATACACAACTAAAC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0010328-'13-14,'13-13,14-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (No Ref, Alt. Stop)

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.353 C2=0.000
Domain overlap (PFAM):

C1:
PF120123=DUF3504=FE(26.1=100)
A:
PF120123=DUF3504=FE(20.0=100)
C2:
PF120123=DUF3504=PD(28.5=79.7)


Main Inclusion Isoform:
FBpp0111770


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0084548, FBpp0300754, FBpp0306778, FBpp0306779


Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
HIGH PSI
([2])
Chicken
(galGal4)
HIGH PSI
Chicken
(galGal3)
HIGH PSI
([4])
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Primers PCR
Suggestions for RT-PCR validation
F:
CATAATGAAGCACTGGAAGCGC
R:
TCCTTGACCATTTTGACGCGG
Band lengths:
242-342
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)